- Gene is segment in the DNA molecule Relation Between DNA, Chromosomes, Genes. On each side of gene is a segment called restriction site
- An enzyme called restriction Enzyme can recognise and cut the restriction site to produce sticky ends.
- DNA strands from 2 diff. organisms are cut by the same restriction enzyme (same restriction enzyme is added to the vector and the DNA containing gene of interest) to produce complementary sticky ends
- DNA fragments from 2 different sources are mixed together with an enzyme called DNA ligase
- The 2 different DNA fragments will bind together by complementary base pairing Rule of Base Pairing. Often sealed together by DNA ligase to form recombinant DNA
Definition
Restriction site is a segment of DNA that is recognised (and cut) by a restriction enzyme